Valery Barstow was meant to become overseer of Vault 88, but the fallout shelter's construction had barely begun when the bombs fell. Barstow was already on-site and got stuck in the vault for more than 200 years, during which she turned into a ghoul. The only thing that kept her sane, and prevented her from turning feral, was her loyalty to Vault-Tec and their cause. She spent most of her life preparing vault experiments. Once saved by the Sole Survivor, she enlists their help in finishing Vault 88's construction and conducting its allotted experiments.
Since Barstow is fanatically dedicated to Vault-Tec's amoral experiments and the mindset behind them, her behavior towards the Sole Survivor changes depending on the way they run her experiments during the Vault-Tec Workshop questline. Going along with the original, ruthless tests will earn her approval. Conversely, choosing the humane options will result in Barstow getting angry and calling the Survivor out on their lack of detachment from their test subjects. If the questline ends with the settlers being happy, such as using all "humane" perimeters in the experiments will result in Valery furiously leaving Vault 88 forever and may later be found dead upon the Survivor's return to the vault.
Killing her at any point in the questline won't negatively impact companion affinity, not even that of compassionate ones like Piper or Curie. Choosing aggressive dialogue options will consistently garner likes from well intentioned companions.
Additionally, she can be convinced to stay through a medium difficulty speech check if at least one of the experiments are ruthlessly carried out, resulting in her becoming the overseer of Vault 88 (though at this point the Sole Survivor is actually the one in charge) and can be commanded to do tasks just like the other settlers.
If the player character decides to kill Barstow, the buildable experiments can be unlocked by looting her body and retrieving a paper marked as a quest item. This actually skips the "testing" phases of the experiments.